Histological and immunohistochemical study on the possible protective effect of curcumin on intestinal ischemia / reperfusion induced lung injury in albino rats

Intestinal ischemia-reperfusion [I/R] injury may lead to severe local and remote tissue injury, especially acute lung injury. Curcumin has been shown to attenuate [I/R] injury in several organ systems. To evaluate the possible protective role of curcumin on acute lung injury induced by intestinal [I/R] in albino rat. Thirty male albino rats were divided equally into control group [Sham-operated], which underwent laparotomy without superior mesenteric artery occlusion. It was classified into two subgroups: subgroup a: which received no treatment, subgroup b: which received curcumin, I/R group where rats were exposed to superior mesenteric artery occlusion for 1 hour followed by 1 hour reperfusion. Third group was I/R and curcumin. Curcumin, was administered in a dose of [100 mg/kg] daily orally for 15 days before experiment. Rats were sacrificed at the end of reperfusion. Lung sections were stained with H and E and Cyclooxygenase -2 immunostain. A morphometric study and statistical analysis were performed. Intestinal I/R caused severe histological changes including massive infiltration of cells in thickened alveolar septa, around bronchioles and congested blood vessels. Curcumin led to obvious regression of induced histological changes by I/R. Significant increase in the mean thickness of inter alveolar septa and the mean area% of Cox-2 immunoexpression were recorded in [I/R] group compared to other groups. Curcumin administration proved a beneficial protective effect on I/R induced acute lung injury, and therefore has potential for clinical use

effect of melatonin on the testes of rats treated with cyclophosphamide: histological and immunohistochemical study

Cyclophosphamide [CP] is used extensively as a chemotherapeutic and an immunosuppressive agent during organ transplantation. However, its clinical utility is limited by adverse actions on the human reproductive system. Melatonin is detected in the human and animal reproductive system, hence assumed to play a useful role in the reproductive cells. To examine the effect of melatonin on the histological and immunohistochemical changes that appear in the cells of the testes of albino rats treated with CP. Forty-two adult male albino rats were classified into group I [control], subgroups IIa and IIIa [CP by an intraperitoneal injection for 2 and 5 weeks, respectively], subgroups IIb and IIIb [CP and melatonin by an intraperitoneal injection for 2 and 5 weeks, respectively], and subgroups IIc and IIIc [CP for 2 and 5 weeks and left for 2 and 5 weeks for recovery, respectively]. The rats were weighed, sacrificed, and the right testes were weighed and processed for paraffin sections. A histological study was carried out using H and E and Masson's trichrome. Immunohistochemical study using the BCL2 antiapoptotic onchoprotein and a morphometric study were carried out. A significant decrease was found in the mean body weight in subgroups IIa, IIIa, and IIIc and in the mean testicular weight in group III. There were distortions in some seminiferous tubules, degeneration of spermatogenic cells, perivascular and intertubular fibrosis, and negative immunoexpression of BCL2. These changes were more marked in group III than in group II. The use of melatonin in subgroups [IIb, IIIb] conferred partial protection against all of the above-mentioned changes. Prolonged administration of CP to rats can induce testicular lesions. Withdrawal of the drug does not ameliorate this effect. The concomitant administration of melatonin with CP led to a partial improvement in testicular lesions induced by CP